Inclusion Criteria:

The following criteria are used to enroll patients in the study before transplant.

• - Patients with neoplastic hematological disorders with indication of allogeneic transplant according to the National Comprehensive Cancer Network (NCCN) or other standard guidelines as follows: - Acute myeloid leukemia [AML] in morphologic complete remission with intermediate/high-risk features (per NCCN criteria) or relapsed disease.

• - Chronic myeloid leukemia [CML] in any chronic phase.

• - Myelodysplastic syndrome [MDS] with intermediate/high risk features or refractory disease (with bone marrow blast count <10%).

• - Acute lymphoblastic leukemia [ALL] in morphologic complete remission with high-risk features or relapsed disease.

• - Negative test for donor-specific antibody within 28 days of starting conditioning regimen.

• - Age Criteria: 19-65 years.

• - Organ Function Criteria: The following organ function testing should be done within 35 days before study registration.

• - Cardiac: Normal left ventricular ejection fraction (LVEF) (50% or above) as measured by MUGA or Echocardiogram.

• - Pulmonary: FVC, FEV1 and DLCO (corrected) should be 50% or above of expected.

• - Renal: serum creatinine level to be <2 mg/dl AND estimated (Cockcroft-Gault formula) or measured (takes priority if done) creatinine clearance (CrCl) must be equal or greater than 70 mL/min/1.73 m2.

• - Hepatic: serum bilirubin 1.5 upper limit of normal (ULN), Aspartate transaminase (AST)/alanine transaminase (ALT) 2.5 ULN, and alkaline phosphatase 2.5 ULN.

• - Performance status: Karnofsky performance score (KPS) or Lansky score: ≥80.

• - Hematopoietic cell transplant comorbidity index (HCT-CI) <3.

Exception may be made on individual cases after discussion with the primary investigator.

• - Consent: All patients must be informed of the investigational nature of this study and given written informed consent in accordance with institutional and federal guidelines.

The following criteria are required within 48 hours prior to infusion of the EAGD T cell product.

• - Absence of uncontrolled infection with sepsis syndrome (e.

g persistent positive blood culture).

• - NO hemodynamic instability (due to sepsis or organ dysfunction) or circulatory volume overload.

• - NO clinically significant organ toxicity that are defined as follows: - Heart failure with subnormal LVEF or clinical fluid overload.

• - Elevated serum creatinine or subnormal creatinine clearance (either estimated or measured).

• - Elevated total bilirubin ≥1.5 upper normal level (unless indirect hyperbilirubinemia attributed to non-hepatic pathology), or elevated liver enzymes (ALT, AST, ALP) >5 x ULN.

• - Hypoxemia requiring oxygen therapy.

• - NO acute graft versus host disease (any grade).

• - Neutrophil engraftment.

Exclusion Criteria:

• - Non-compliant patients.

• - No appropriate caregivers identified.

• - Uncontrolled medical or psychiatric disorders which may preclude patients to undergo clinical studies (Discretion of the attending physician).

• - Active central nervous system (CNS) neoplastic involvement.

• - Morbid obesity with body mass index >35 (borderline cases may be considered on case-by-case basis after discussion with the primary investigator).

• - Patients with known allergy to DMSO.

• - HIV1 (Human Immunodeficiency Virus-1) or HIV2 positive.

• - Pregnant or breastfeeding women.

Many patients with hematological malignancies require a bone marrow transplant for curative treatment. A matched sibling donor is optimal but may not be available. Therefore, a partially matched family member (haploidentical) may be a viable alternative. The incidence of graft vs.#46; host disease, however, can become more of a significant, even fatal, factor with partial matches. T-cells have been shown to be the key player in the post-transplant immune phenomena. The majority of T-cells are composed of alpha beta T-cells with a small minority of gamma delta T-cells, which are known to have the unique ability to kill malignant cells without antigen recognition. This study proposes to extract, concentrate, and activate gamma delta T-cells from the peripheral blood to provide innate anti-tumor effect with minimal risk of GVHD. Safety and impact and/or the rate of GVHD will be evaluated.

Arms

Experimental: EAGD T-cell infusion (Phase I)

Peripheral blood is collected by leukapheresis from the donor, expanded and activated on CliniMACS-Prodigy, further depleted of alpha beta T-cells using the CliniMACS Alpha Beta T-Cell Depletion System, which leaves a gamma delta T-cell rich product. This product is then infused into the recipient at either 1, 3, or 10 x 1,000,000 cells/kg concentrations depending upon the cohort.

Experimental: EAGD T-cell infusion (Expansion)

Peripheral blood is collected by leukapheresis from the donor, expanded and activated on CliniMACS-Prodigy, further depleted of alpha beta T-cells using the CliniMACS Alpha Beta T-Cell Depletion System, which leaves a gamma delta T-cell rich product. This product is then infused into the recipient at the maximum tolerated dose as determined from Phase I.

Interventions

Drug: - EAGD T-cell infusion (Phase I)

The Alpha Beta (α/β) T-Cell Depletion System utilizes the CliniMACS instrument to yield a gamma delta (γδ) enriched cell therapy product.

Drug: - EAGD T-cell infusion (Expansion)

The Alpha Beta (α/β) T-Cell Depletion System utilizes the CliniMACS instrument to yield a gamma delta (γδ) enriched cell therapy product.